GsMTx4 i35-amino acid peptide ine mana disulfide bond ayo anoumba cysteine knot motif, inova yakajairika chimiro chezhinji spider venom peptides inopa kugadzikana uye kujeka.Iyo mashandiro ekuita kweGsMTx4 haina kujekeswa zvizere, asi inofungidzirwa kuti inosunga kune extracellular kana transmembrane domains ye cationic MSCs uye inovharira kuvhurika kwavo kwepore kana geting nekuchinja kuwirirana kwavo kana membrane tension.GsMTx4 yakaratidzwa kuvharidzira akati wandei cationic MSCs ine sarudzo dzakasiyana uye potency.Semuyenzaniso, GsMTx4 inovharisa TRPC1 ine IC50 ye0.5 μM, TRPC6 ine IC50 ye0.2 μM, Piezo1 ine IC50 ye0.8 μM, Piezo2 ine IC50 ye0.3 μM, asi haina mhedzisiro inokwira kusvika TRP411 μM.(Bae C et al 2011, Biochemistry)
GsMTx4 yakashandiswa sechishandiso chemishonga yekudzidza basa uye kudzora kweiyo cationic MSCs mumhando dzakasiyana siyana dzemasero uye matishu.Mimwe yemienzaniso yacho ndeiyi:
GsMTx4 inogona kuvhara maMSCs anoshandiswa nekutambanudza mune astrocytes, masero emwoyo, masero akatsetseka emhasuru uye skeletal tsandanyama masero.Astrocyte masero akaita senyeredzi anotsigira uropi uye musana.Masero emwoyo ndiwo masero anoumba tsandanyama yemwoyo.Smooth muscle cells ndiwo masero anodzora kufamba kwenhengo dzakaita sedumbu netsinga dzeropa.Skeletal muscle cells ndiwo masero anogonesa kufamba kwemuviri nekuzvidira.Nekuvhara maMSCs mumaseru aya, GsMTx4 inogona kushandura midziyo yavo yemagetsi, mazinga ecalcium, contraction uye kuzorora, uye gene expression.Shanduko idzi dzinogona kukanganisa mashandiro anoita masero aya kana mumamiriro ezvirwere (Suchyna et al., Nature 2004; Bae et al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMTx4 inogonawo kuvhara mhando yakakosha yeMSC inonzi TACAN, iyo inobatanidzwa mumhinduro yemarwadzo.TACAN inzira inoburitswa mumaseru etsinga anonzwa kurwadziwa.TACAN inobatiswa nemakanika stimuli, senge kudzvanywa kana kupinza, uye inokonzera marwadzo.GsMTx4 inogona kuderedza basa reTACAN uye kuderedza maitiro ekurwadziwa mumhando dzemhuka dzemarwadzo emagetsi (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Nature Communications 2013)
GsMTx4 inogona kudzivirira astrocytes kubva kune chepfu inokonzerwa nemorekuru inonzi lysophosphatidylcholine (LPC), inova lipid murevereri inokonzera kukanganisa kuuropi uye musana.LPC inogona kumisa MSCs mune astrocytes uye kuita kuti vatore yakawandisa calcium, izvo zvinotungamira kune oxidative kusagadzikana uye sero kufa.GsMTx4 inogona kudzivirira LPC kubva ku activate MSCs mune astrocytes uye kuvadzivirira kubva kune chepfu.GsMTx4 inogonawo kuderedza kukuvadzwa kwehuropi nekuvandudza kushanda kwetsinga mumakonzo akaiswa jekiseni neLPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 inogona kuenzanisa neural stem cell kusiyanisa nekuvharira imwe mhando yeMSC inonzi Piezo1, iyo inoratidzwa muneural stem masero.Neural stem masero masero anogona kugadzira neuroni itsva kana mamwe marudzi emasero europi.Piezo1 igwara rinoitwa nemechanical cues kubva kunharaunda, sekuoma kana kudzvanywa, uye rinopesvedzera kuti neural stem masero anosarudza rudzii rwesero.GsMTx4 inogona kupindira nePiezo1 chiitiko uye kuchinja neural stem cell kusiyanisa kubva kune neurons kuenda kune astrocytes (Pathak et al., Journal of Cell Science 2014; Lou et al., Cell Reports 2016)
Isu tiri mugadziri wepolypeptide muChina, tine makore akati wandei ehunyanzvi mukugadzira polypeptide.Hangzhou Taijia Biotech Co, Ltd inyanzvi yepolypeptide mbishi kugadzira, iyo inogona kupa makumi ezviuru zvepolypeptide mbishi zvinhu uye inogona zvakare kugadzirwa zvinoenderana nezvinodiwa.Hunhu hwezvigadzirwa zvepolypeptide hwakanakisa, uye kuchena kunogona kusvika 98%, iyo yave ichizivikanwa nevashandisi pasi rese.Welcome to consult us.
