Pepstatin pentapeptide, inowanzoitika aspartyl protease inhibitor, inogona kuvharidzira aspartic protease uye acid protease yezvipembenene zvakasiyana-siyana.Pepstatin inonyanya kuvezwa neStreptomyces marudzi uye inogadzirwa neStreptomyces.Inogona kurambidza pepsin, pepsin D uye angiotensin-inoburitsa enzyme, uye ine mhedzisiro yekurapa pagastric ulcer, renal hypertension, arthritis, carrageenan edema nezvimwe zvirwere.
Pepstatin inhibitor yakasimba yeaspartyl proteases yakadai se pepsin, cathepsin D, uye renin.Iyi pentapeptide yechisikigo yakaparadzaniswa kubva ku actinomycetes yaive yemakore akawanda classic renin inhibitor in vitro.Pepstatin haina kunangana nerenin uye haina kunyungudika mumvura.Pepstatin ine zvisingawanzo γ amino acid statin iyo inogona kutsiva maviri amino acids pane scissile bond yeprotein substrate uye inovhara substrate cleavage nekuda kwekufananidzwa kwechimiro. kune shanduko yemamiriro e peptide bond hydrolysis ne aspartyl proteases.
Apo mapuroteni anobviswa mumasero akatyoka, maprotease anogona kubudiswa, ayo anoda kukurumidza kudziviswa kuchengeta mapuroteni kuti asashatiswa.Mukuita kubviswa kweprotein, protease inhibitors inoda kuwedzerwa kudzivirira proteolysis.Protease inhibitor inoreva zvakanyanya kune chinhu chinosunga kune mamwe mapoka panzvimbo inoshanda yemamorekuru eprotease, kuitira kuti basa reprotease ridzike kana kunyangarika, asi risingadzore puroteni yeenzyme.Kunzwa kwemapuroteni akasiyana kune akasiyana mapuroteni akasiyana, saka zvinodikanwa kugadzirisa kusangana kweakasiyana maprotease.Nekuti kunyungudika kweprotease inhibitor mumvura kwakadzikira zvakanyanya, zvinofanirwa kucherechedzwa kuti protease inhibitor inofanira kusanganiswa zvizere mubuffer kuderedza kunaya kweprotease inhibitor.Pepstantin A inogona kuvharidzira acid proteases akadai pepsin, angiotensin, collagenase, cathepsin D uye chymosin.
Pepstatin A inhibitor ye cathepsin d uye e.Mushure meHEK293 masero akabatwa nemhando dzakasiyana dzepepstatin A kwemaawa makumi maviri nemana, kutaura kweLC3Ⅱ uye p62 kwakaonekwa.Mhedzisiro yacho yakaratidza kuti pepstatin A inogona kuwedzera zvakanyanya kutaura kweLC3Ⅱ uye P62 (P<0.05) nenzira inoenderana nedosi.20μ g/ml pepstatin A yakashandiswa kurapa HEK293 panguva dzakasiyana dzenguva, uye mhedzisiro yenguva dzakasiyana dzenguva pakutaura kweLC3II uye p62 yakaonekwa.Mhedzisiro yacho yakaratidza kuti pepstatin A inogona kusimudza-kutonga kutaura kweLC3II uye p62 nenzira inoenderana nenguva.
Isu tiri mugadziri wepolypeptide muChina, tine makore akati wandei ehunyanzvi mukugadzira polypeptide.Hangzhou Taijia Biotech Co, Ltd inyanzvi yepolypeptide mbishi kugadzira, iyo inogona kupa makumi ezviuru zvepolypeptide mbishi zvinhu uye inogona zvakare kugadzirwa zvinoenderana nezvinodiwa.Hunhu hwezvigadzirwa zvepolypeptide hwakanakisa, uye kuchena kunogona kusvika 98%, iyo yave ichizivikanwa nevashandisi pasi rese.Welcome to consult us.
